MFO-6 Microbiological Examination of Egg Products and of Liquid Eggs
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B9FFC24C77E448D29DBDB6D60763758C |
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0.02 |
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页数： |
6 |
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日期： |
2012-3-2 |
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Published by: POLYSCIENCE PUBLICATIONS, P.O. Box 1606, Station St-Martin, Laval, Quebec,Canada H7V 3P9. TEL.: 1-800-840-5870. FAX: (450) 688-1930.,Government of Canada Gouvernement du Canada,Official Method MFO-6,November 30, 1981,HEALTH PROTECTION BRANCH,OTTAWA,MICROBIOLOGICAL EXAMINATION OF EGG PRODUCTS AND OF LIQUID EGGS,1. APPLICATION,This method shall be used for the determination of bacteria of the genus Salmonella in egg products and,in liquid eggs, in accordance with Section B.22.033 of the Food and Drug Regulations.,2. SAMPLING,2.1 Definition of Terms,2.1.1 Lot: A batch or production unit which may be identified by the same code. When there is,no code identification, a lot may be considered as (a) that quantity of product produced,under essentially the same conditions, at the same establishment and representing no more,than one day's production; or, (b) the quantity of the same kind of product from one and the,same manufacturer available for sampling at a fixed location.,2.1.2 Sample: The sample units (subsamples) taken per lot for analysis.,2.1.3 Sample Unit: Usually a consumer size container of the product, and should consist of a,minimum of 100 g. A sample unit is often referred to as a subsample.,2.1.4 Analytical Unit: That amount of product withdrawn from the sample unit for analysis.,2.2 Collection of Samples,2.2.1 A sample, consisting of ten sample units drawn at random from each lot, shall be taken.,2.2.2 Each sample unit shall contain at least 100 g or ml.,2.2.3 Collect original unopened containers wherever possible.,2.2.4 More than one sample unit may be collected from large institutional or bulk containers when,the total number of sample units required exceeds the number of containers in the lot.,When the lot consists of containers smaller than 100 g, a sample unit will consist of more,than one container (e.g., four 25 g containers in each sample unit).,2.2.5 Employ aseptic techniques in collecting the sample units when sampling from bulk. Place each,collected sample unit into a separate sterile container.,2.2.6 Keep unfrozen sample units refrigerated (0-5oC), and frozen sample units frozen during,transport.,MFO- 6,November 30, 1981,-2-,Published by: POLYSCIENCE PUBLICATIONS, P.O. Box 1606, Station St-Martin, Laval, Quebec,Canada H7V 3P9. TEL.: 1-800-840-5870. FAX: (450) 688-1930.,3. PROCEDURE,The 10 sample units shall be analyzed individually or as one or more composite(s) for determining the presence,of bacteria of the genus Salmonella.,The test shall be carried out in accordance with the following instructions:,3.1 Handling of Sample Units,3.1.1 Keep unfrozen sample units refrigerated (0-5oC), and frozen sample units frozen in the,laboratory prior to analyzing them.,3.1.2 Analyze the sample units as soon as possible after they have been received in the laboratory.,3.2 Preparation of Media,The following media, which are to be prepared and sterilized according to the manufacturer's instructions,shall be used:,(1) Nutrient Broth (NB),(2) Selenite Cystine (SC) broth,(3) Tetrathionate Brilliant Green (TBG) broth,(4) Bismuth Sulfite (BS) agar,(5) Brilliant Green Sulfa (BGS) agar,(6) MacConkey Agar (MA),(7) Triple Sugar Iron (TSI) agar,(8) Lysine Iron (LI) agar,(9) Christensen's Urea (CU) agar,(10) Nutrient Agar (NA),3.3 Non-Selective Enrichment (Pre-enrichment),3.3.1 Thaw frozen sample units. Do not allow the temperature of any sample unit to exceed 45oC.,3.3.2 Withdraw a 25 g analytical unit from each 100 g sample unit. When a sample unit consists of,more than one container mix the contents of each container of the sample unit aseptically prior,to obtaining the 25 g analytical unit. The analytical units may be composited. Since compositing,presents difficulties in preparation and disposal of the materials, exercise care in handling bulk,preparations.,3.3.3 Suspend the individual analytical units or the composite unit(s) in nine times their weight of NB,in a pre-warmed, sterile blender jar.,3.3.4 Blend the mixture at low speed for two min.,3.3.5 Check the pH of each blended analytical or composite unit. If the pH is outside the range of 6.0,- 7.0, adjust to 7.0 with either sterile NaOH or HCl.,3.3.6 Inoculate NB with a known culture of Salmonella and subsequently make transfers to all other,media used in the analysis. This is the positive media control. Set up a negative control by,incubating appropriate uninoculated media during each step of the analysis.,MFO- 6,November 30, 1981,-3-,3.3.7 Incubate the inoculated pre-enrichment broth(s) and the controls at 35oC ± 0.5o for 18-24 hr. In,no circumstances shall the incubation be prolonged for more than 24 hr.,……
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